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US 5,631,140 AGranted
Method for the quantitative determination of glycated proteins
AssigneeBoehringer Mannheim GmbH
InventorsUwe Kobold, Doris Renauer, Andreas Finke, Johann Karl
Life of this patent
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Abstract
Method for the quantitative determination of glycated protein in particular haemoglobin and HbA1c, wherein the respective sample is firstly contacted with a proteolytic enzyme and subsequently a non-immunological chromatographic separation process is carried out.
Claims19
A method for the determination of a glycated protein relative to non-glycated protein in an aqueous sample, comprising:
- reacting the sample with a proteolytic enzyme to form a peptide mixture comprising an amount of glycated peptides and an amount of non-glycated peptides,
- separating said glycated peptides and said non-glycated peptides in said peptide mixture, and
- determining said glycated protein relative to said non-glycated protein by measuring the amount of said glycated peptides and the amount of said non-glycated peptides and comparing the amount of said glycated peptides with the amount of said non-glycated peptides.
Dependencies are inferred from the claim text.