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AssigneeIgen, Inc.
InventorsMark T. Martin, Rodger G. Smith, Michael J. Darsley, David M. Simpson, Gary F. Blackburn
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Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.
A process for selecting a recombinant virus population capable of expressing a surface bound catalytic moiety, said process comprising the steps of:
A process for increasing the proportion of catalytic moieties to non-catalytic moieties in a first sample comprising a population of recombinant viruses suspected of including viruses capable of expressing the catalytic moiety, said process comprising the steps of:
Dependencies are inferred from the claim text.