Process for the detection of complexed cathepsin G and .alpha.-1-antichymotrypsin
AssigneeBehringwerke Aktiengesellschaft
InventorsKlaus Havemann, Hans-Heinrich Heidtmann
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Abstract
The present invention relates to a process for determining complexes of .alpha.-1-antichymotrypsin and cathepsin G in a sample comprising adsorbing the cathepsin G portion of the complex to a solid phase coated with non-specific binding protein or gelatin, and detecting the .alpha.-1 portion of the complex with a detectably labelled anti-.alpha.-1-antichymotrypsin antibody. A diagnostic kit comprising the solid phase coated with non-specific binding protein or gelatin and the labelled anti-.alpha.-1-antichymotrypsin antibody is also provided.
Claims11
A process for detecting complexes of .alpha.-1-antichymotrypsin and cathepsin G in a sample, comprising:
- (a) providing a solid phase wherein surface of the solid phase is saturated with a coating capable of adsorbing said cathepsin G but not said .alpha.-1-antichymotrypsin and wherein said coating is selected from the group consisting of a non-specific binding protein and gelatin;
- (b) contacting the sample to the solid phase of step (a) to adsorb said complexes;
- (c) contacting the solid phase of step (b) with an antibody which specifically binds to the .alpha.-1-antichymotrypsin present in the adsorbed complexes;
- and,
- (d) detecting bound antibody to detect the complexes in the sample.
A process for quantitating the amount of complexes of .alpha.-1-antichymotrypsin and cathepsin G in a sample, comprising:
- (a) providing a solid phase wherein surface of the solid phase is saturated with a coating capable of adsorbing said cathepsin G but not said .alpha.-1-antichymotrypsin and wherein said coating is selected from the group consisting of a non-specific binding protein and gelatin;
- (b) contacting the sample to the solid phase of step (a) to adsorb said complexes;
- (c) contacting the solid phase of step (b) with an antibody which specifically binds to the .alpha.-1-antichymotrypsin present in the adsorbed complexes;
- (d) measuring the amount of antibody bound to the solid phase of step (c);
- and,
- (e) determining the amount of said complexes in the sample by comparing the amount of bound antibody measured in step (d) with a calibration curve constructed by measuring the amount of bound antibody produced by calibrators comprising known quantities of said complexes assayed according to steps (a) through (d).
Dependencies are inferred from the claim text.