Materials and methods for the detection of Mycobacterium tuberculosis
AssigneeAbbott Laboratories
InventorsGregor W. Leckie, Alan H. Davis, Ingrid E. Semple-Facey, Matthew T. Manlove, Natalie A. Solomon
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Abstract
The present invention is directed to oligonucleotides useful in detection, e.g., by ligase chain reaction (LCR) of target DNA from Mycobacterium tuberculosis. The present invention is also directed to methods of detecting target DNAs from Mycobacterium tuberculosis.
Claims11
A composition for detecting a target DNA sequence from Mycobacterium tuberculosis, said composition comprising a probe pair wherein said probe pair is characterized by:
- a) said probe pair consists of two oligonucleotide probes each having between about 10 and about 30 nucleotides wherein each of said two oligonucleotide probes is complementary to different regions of said target DNA and are capable of hybridizing with a sequence within said target DNA sequence and wherein said target DNA sequence is SEQ ID NO. 21 or its complement;
- and
- b) said probe pair does not detect Mycobacterium avium.
A kit useful in the detection of target DNA from Mycobacterium tuberculosis, said kit comprising one or more containers containing a probe pair consisting of two oligonucleotide probes each having between about 10 and about 30 nucleotides wherein
- a) each of said two oligonucleotide probes is complementary to different regions of said target DNA and are capable of hybridizing with a sequence within said target DNA sequence wherein said target DNA SEQ ID NO. 21 or its complement;
- and
- b) said probe pair does not detect Mycobacterium avium.
Dependencies are inferred from the claim text.