Biologic bioadhesive compositions containing fibrin glue and liposomes, methods of preparation and use

A process for forming a flexible fibrin glue and liposome composition in the form of a film or membrane comprised of said fibrin glue in which said liposomes are embedded, the film or membrane used for coating or implantation in vitro or in vivo, comprising:

• a) preparing said fibrin glue and liposome composition comprising:
• i) virus-inactivated fibrinogen at a concentration of about 10 to 90 mg/mL;
• ii) virus-inactivated thrombin at a concentration of about 1 to 200 U/mL;
• and
• iii) liposomes at a concentration of about 1% to 20%, by volume;
• b) mixing the components of step a);
• c) sprayably applying the composition onto a substrate;
• the substrate providing a surface onto which the composition temporarily adheres and gels to form a flexible film or membrane comprising the gelled fibrin glue and liposome composition;
• d) removing from the substrate surface the film or membrane comprising the fibrin glue and liposome composition after said composition has gelled.

A method for hemostatically sealing and healing a wound or a surgical incision and delivering to the wound or incision medicaments, comprising:

• a) preparing a liposome-containing fibrin glue composition, comprising in admixture:
• (i) virus-inactivated fibrinogen present in said composition at a concentration of 10 to 90 mg/mL;
• (ii) virus-inactivated thrombin present in said composition at a concentration of 1 to 200 U/mL;
• (iii) liposomes present in said composition at a concentration of about 1% to 20%, by volume, said liposomes formulated to contain one or more medicaments within their interior aqueous phases;
• and
• (iv) calcium present in said composition at a concentration of about 1 to 30 mM;
• b) administering said composition to the surface of said wound or incision, wherein coagulation of said glue forms a medium for entrapping said liposomes at said site, embeds said liposomes in said glue, and allows sustained release of said liposome contents delivered within said wound or incision.