Compositions, methods and apparatus for ultrafast electroseparation analysis

A method for detecting the absence, presence or concentration of an analyte in a sample, the method comprising the steps of:

• (a) providing an electroseparation channel comprising
• (i) a first zone, said first zone comprising a first binding partner which binds to a first binding site on an analyte to be detected, said first binding partner comprising a detectable moiety, and
• (ii) a second zone, said second zone comprising a second binding partner which binds to a second binding site on said analyte, said second binding partner comprising a charge-modifying moiety;
• (b) disposing a sample within said electroseparation channel;
• (c) contacting said sample with said first and second zone so that said analyte, if present in said sample, will form a three-membered complex comprising analyte, first binding partner and second binding partner;
• (d) separating said three-membered complex, if present, from unbound first binding partner;
• and,
• (e) detecting said complex.

A method for electroseparation analysis of a mixture in a channel, the method comprising the step of:

• electrically separating a mixture comprising:
• (a) a sample;
• (b) a first binding partner which binds to a first binding site on an analyte, said first binding partner comprising a detectable moiety;
• and,
• (c) a second binding partner which binds to a second binding site on said analyte, said second binding partner comprising a charge-modifying moiety, wherein the analyte, if present in said sample, will form a three-membered complex with the first binding partner and the second binding partner.