Promoters for gene expression
AssigneeAllelix Biopharmaceuticals Inc.
InventorPeter A. Lagosky
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Abstract
The present invention provides a novel expression system for the production of protein in bacterial hosts. The system utilizes novel promoters that are highly efficient in initializing transcription and therefore enhance protein yield. The promoters comprise the -35 region of the consensus E. coli promoter, the -10 region of the lppP-5 promoter and a spacer between these two regions derived from either the lpp or lacUV5 promoters.
Claims20
A recombinant DNA construct which expresses a protein in a bacterial host cell, the construct comprising a coding region for the protein operably linked with a control region comprising a promoter which promotes expression of said protein in the host cell, wherein the promoter comprises a DNA sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2.
Dependencies are inferred from the claim text.