Plant transformation by gene transfer into pollen
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsJames A. Saunders, Benjamin F. Matthews
Life of this patent
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Abstract
Plant germplasm is transformed with foreign DNA by introducing the DNA into pollen grains by a technique such as electroporation, mating ova of the desired plant line with the transformed pollen, and selecting for the transformed germplasm. The germinating pollen, resultant seed, and the progeny can each be screened for expression of the foreign gene. The transformed pollen can be used as a vector for introducing the foreign DNA into plant lines of similar or dissimilar origin, including both monocots and dicots.
Claims12
A method for transforming a plant with a foreign DNA comprising:
- a. introducing said foreign DNA into pre-germinated pollen by electroporation, wherein said foreign DNA is in an expression vector and is capable of participating in a recombination event with the native DNA in said pollen and wherein said pollen is capable of fertilizing ova of the plant;
- b. fertilizing ova of the plant;
- and
- c. selecting for germplasm which is transformed with said foreign DNA.
A method for introducing foreign DNA into a plant comprising:
- a. introducing said foreign DNA into pre-germinated pollen by electroporation, wherein said pollen is capable of fertilizing ova of the plant;
- b. fertilizing ova of the plant;
- and
- c. selecting for expression of said foreign DNA.
Dependencies are inferred from the claim text.