Molecular farming
AssigneeCalgene, Inc.
InventorsRobert M. Goodman, Vic C. Knauf, Catherine M. Houck, Luca Comai
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Abstract
Novel constructs are provided for expression of physiologically active mammalian proteins in plant cells, either in culture or under cultivation. The constructs provide a promoter functional in a plant host, a structural gene coding for mammalian protein and a terminator functional in a plant host. The construct is introduced into a plant cell to become integrated into the plant genome for expression in the plant cells or plants. The plant cells may be harvested and the mammalian protein isolated in physiologically active form.
Claims8
A method for producing a mammalian peptide which comprises:
- growing tobacco plant cells containing an integrated sequence comprising,
- a first expression cassette having in the direction of transcription (1) a transcriptional and translational initiation region functional in said plant cells, (2) a structural gene coding for said mammalian peptide, and (3) a termination region,
- whereby said structural gene is expressed to produce said mammalian peptide.
In a method for the production of a foreign peptide in a transgenic dicotyledonous plant cell containing an expression cassette,
- said expression cassette having in the direction of transcription (1) a transcriptional and translational initiation region functional in said plant cell, (2) a structural gene coding for said foreign peptide, and (3) a termination region,
- the improvement characterized by:
- a structural gene coding for a mammalian peptide as said structural gene coding for said foreign peptide in said expression cassette.
A method for producing an interferon which comprises:
- growing dicotyledonous plant cells containing an integrated sequence comprising,
- an expression cassette having in the direction of transcription (1) a transcriptional and translational initiation region functional in said plant cells, (2) a structural gene coding for an interferon, and (3) a termination region,
- whereby said structural gene is expressed to produce said interferon.
A method for producing a mammalian peptide which comprises:
- growing dicotyledonous plant cells containing an integrated sequence comprising,
- a first expression cassette having in the direction of transcription (1) a transcriptional and translational initiation region functional in said plant cells, (2) a structural gene coding for said mammalian peptide, and (3) a termination region,
- and a second expression cassette comprising in the direction of transcription (1) a transcriptional and translational initiation region functional in said plant cells, (2) a marker gene encoding an enzyme which allows for selection of plant cells expressing said gene, and (3) a termination region,
- whereby said structural gene is expressed to produce said mammalian peptide.
Dependencies are inferred from the claim text.