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US 5,629,167 AGranted
Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
AssigneeBiocine S.p.A.
InventorGiulio Ratti
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Abstract
A new recombinant form of the plasmid-encoded protein pgp3 from C. trachomatis, serotype D, was purified by ion exchange column chromatography and shown to be suitable for quantitative immunoassy on clinical samples in an ELISA format.
Claims2
An enzyme-linked immunosorbent assay for the detection of anti-Chlamydia trachomatis pgp3 antibody in a patient sample comprising the following steps:
- (a) contacting the patient sample with a recombinant Chlamydia trachomatis pgp3 protein consisting of the amino acid sequence of native pgp3 protein bound to a solid support for a time sufficient to effect the binding of antibody to said bound protein;
- (b) detecting the binding of said antibody to said bound protein;
- (c) comparing the amount of antibody bound in step (b) to a control, wherein an increase over the control indicates the presence of anti-Chlamydia trachomatis pgp3 antibody.
Dependencies are inferred from the claim text.