Calcineurin inhibitory compounds and anchoring protein
AssigneesICOS Corporation, State of Oregon, Acting by and Through the Oregon State Board of Higher Education on Behalf of the Oregon Health Sciences University
InventorsJohn D. Scott, Vincent M. Coghlan, Monique L. Howard, William M. Gallatin
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Abstract
The present invention provides compositions and methods useful for isolating calcineurin as well as inhibiting calcineurin activity. The compositions are peptides that contain regions that are homologous to calcineurin-binding regions of AKAP 79. Also provided are methods for determining if a cell contains a calcineurin-binding and PKA-binding anchoring protein that are useful for identifying additional proteins that bind both calcineurin and PKA.
Claims1
A method for determining if a cell contains a calcineurin-binding and PKA-binding anchoring protein, the method comprising the steps of:
- lysing the cell to form a lysate;
- incubating the lysate with a solid support, the solid support having calcineurin molecules immobilized thereon;
- washing the solid support;
- contacting the solid support with a labeled PKA regulatory subunit that can bind an anchoring protein;
- washing from the solid support regulatory subunit that did not bind;
- detecting regulatory subunit remaining bound on the solid support;
- and
- determining therefrom the presence of a calcineurin-binding and PKA-binding protein in the cell.
Dependencies are inferred from the claim text.