Methods for characterizing phosphorothioate oligonucleotides
AssigneeIsis Pharmaceuticals, Inc.
InventorTadeusz K. Wyrzykiewicz
Life of this patent
Estimating the term…
Abstract
This invention presents novel methods for desulfurizing and sequencing phosphorothioate oligonucleotides. These methods allow easy access to the analysis of phosphorothioate oligonucleotides because these methods may be performed as a final step in oligonucleotide synthesis. The methods of the invention will permit rapid characterization of oligonucleotides and may be easily adapted to characterizing pharmaceutical formulations.
Claims24
A method for desulfurizing a phosphate protected phosphorothioate oligonucleotide synthesized on a solid phase support comprising the steps of:
- a) removing the phosphate protecting groups before removal of said oligonucleotide from said solid phase support;
- and
- b) treating said deprotected oligonucleotide with an iodine solution comprising at least about 15 equivalents of iodine per sulfur atom of the oligonucleotide.
A method for desulfurizing a cyanoethyl protected phosphorothioate oligonucleotide bound to a solid support comprising treating the bound oligonucleotide with an iodine solution for a period of time effective to desulfurize said oligonucleotide.
A method for desulfurizing a phosphate protected phosphorothioate oligonucleotide synthesized on a solid phase support comprising the steps of:
- a) removing the phosphate protecting groups before removal of said oligonucleotide from said solid phase support;
- and
- b) treating said deprotected oligonucleotide with an iodine solution comprising at least about 10 equivalents of iodine per sulfur atom of the oligonucleotide.
Dependencies are inferred from the claim text.