Method of forming an iodine based germicide composition

A method of forming an iodine based germicide in situ in an aqueous based medium having a stabilized concentration of available iodine comprising the steps of combining a source of peroxide, a source of iodine ions, a peroxidase that falls within the class of peroxidase in the Enzyme Commission identification No. E.C. 1.11.1.7. and an iodine sequestrant selected from the group consisting of:

• polyvinylpyrrolidone, block copolymers of ethylene oxide and propylene oxide, alkylphenol ethoxylates, tetra-functional block copolymers derived from the sequential addition of propylene oxide and ethylene oxide to ethylenediamine, copolymers of ethylene oxide and propylene oxide, and ethoxylated alcohols that range from 8 to 18 carbon atoms in length so as to form a non-reactive enzymatic composition, admixing said composition with an aqueous based medium to cause an enzymatic catalyzed reaction with said iodine sequestrant being added to said composition at a time prior to the equilibration of the enzymatic reaction such that the concentration of available iodine in said composition increases as compared to an identical composition without an iodine sequestrant.