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OwnerStaffan Birnbaum, Jonas Johansson +6inventors
InventorsStaffan Birnbaum, Jonas Johansson, Per-Olof Larsson, Akiyoshi Miyabayashi, Klaus Mosbach, Staffan Nilsson, Sune Svanberg, Karl-Gustav Wahlund
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The present invention comprises a method Of detecting and quantifying components separated by capillary electrophoresis or other techniques using thin capillaries in the separation. The method of the present invention comprises irradiating the whole or large parts of the capillary at short time intervals and detecting the fluorescence originating from the sample components, preferably by a CCD detector (charge coupled device). Thereby, an instantaneous image of the progress of the separation process is obtained. The present invention further comprises an apparatus for performing this detection.
A method of analyzing a sample by separating components of said sample in a thin capillary, irradiating said capillary, and detecting light selected from the group consisting of light emitted by and light absorbed by said components of said sample, comprising detecting said light at short intervals during said separating simultaneously over the whole or at least a large part of said capillary to create successive momentary images of the separation pattern and thereby the progress of the separation, thereby permitting termination of said separating when a desired separation of said components of said sample is achieved.
An apparatus for sample analysis by monitoring the pattern of separation of sample components and thereby the progress of separation thereof, comprising a separation capillary, a light source for irradiating said separation capillary, and a detector for detecting light selected from the group consisting of light emitted from and light emitted by said sample components, wherein said light source irradiates the whole or at least a large part of said separation capillary, and wherein said detector is an imaging detector arranged to register data at short intervals during the separation light emitted from or absorbed by said sample components depending on their spectral characteristics simultaneously over the whole or at least a large part of said separation capillary to create successive momentary images of said separation pattern and thereby the progress of said separation.
Dependencies are inferred from the claim text.