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US 5,627,277 AGranted
Method for analyzing oligonucleotide analogs
AssigneeHybridon, Inc.
InventorsAharon S. Cohen, Andre Bourque, Maria Vilenchik
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Abstract
Disclosed is a substrate useful for separating unmodified and modified mononucleotides and oligonucleotides. The substrate includes at least 12% (weight:volume) polymer in at least 5M urea and at least 32% (volume:volume) organic solvent, the organic solvent being a chemically stable liquid at room temperature and having a dielectric constant of at least 20. Also provided is a method of separating unmodified and modified mononucleotides and/or oligonucleotides utilizing this substrate.
Claims25
A method of separating modified mononucleotides and/or modified oligonucleotides, comprising the steps of:
- (a) providing, in a capillary, a substrate comprising at least 12% (weight:volume) of a polymer suitable for use in high performance capillary electrophoresis, at least 5M urea and at least 32% (volume:volume) organic solvent, wherein the organic solvent is a chemically stable liquid at room temperature and has a dielectric constant of at least 20;
- (b) contacting the substrate with the mononucleotides and/or oligonucleotides to be separated;
- (c) applying an electric field greater than 200 volts/cm across the substrate in the capillary to separate the mononucleotides and/or oligonucleotides;
- and
- (d) detecting the separated mononucleotides and/or oligonucleotides.
2 more elements
Dependencies are inferred from the claim text.