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US 5,627,268 AGranted
Hemoglobin comprising globin fusion proteins
AssigneeDNX Biotherapeutics
InventorsRamesh Kumar, Ajay Sharma, Anastasia M. Khoury-Christianson
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AssigneeDNX Biotherapeutics
InventorsRamesh Kumar, Ajay Sharma, Anastasia M. Khoury-Christianson
Estimating the term…
The present invention relates to a novel method for making a predetermined, desired peptide in transgenic animals and can be advantageously used for the production of large quantities of the desired peptide. More particularly, the invention concerns the engineering of a transgenic animal having an artificial gene, which is controlled by globin locus control region (LCR) and which encodes a fusion protein, in which the desired peptide is joined via a cleavable peptide linker to a globin polypeptide. The erythrocytes of the transgenic animal express the fusion globin which is incorporated into hemoglobin produced by the host cell. The desired peptide can be obtained from a hemolysate of the red cells of the transgenic animals by enzymatic or chemical cleavage at the linker.
A hemoglobin comprising at least one fusion protein comprising a globin polypeptide and a predetermined peptide having less than about 65 amino acids, wherein the globin polypeptide is attached to the predetermined peptide through a selectively cleaveable peptide bond and wherein the net charge of the selectively cleaveable peptide bond and the predetermined protein is -4 to +5.
Dependencies are inferred from the claim text.