Cytokine designated elk ligand
AssigneeImmunex Corporation
InventorsStewart Lyman, M. Patricia Beckmann, Peter R. Baum
Life of this patent
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Abstract
elk ligand (elk-L) polypeptides as well as DNA sequences, vectors and transformed host cells useful in providing elk-L polypeptides. The elk-L polypeptide binds to a cell surface receptor that is a member of the tyrosine kinase receptor family.
Claims10
A substantially homogeneous purified mature elk-L protein, wherein said elk-L protein binds elk, wherein said elk-L protein comprises an amino acid sequence that is at least 80% identical to a sequence selected from the group consisting of amino acids 1-322 of SEQ ID NO:2 and amino acids 1-213 of SEQ ID NO:2.
A purified elk-L protein which binds elk, wherein said elk-L protein comprises modification(s) selected from the group consisting of:
- (a) inactivation of N-glycosylation site(s);
- and
- (b) inactivation of KEX2 protease processing site(s);
- and
- wherein the amino acid sequence of said elk-L protein is otherwise identical to amino acids 1-322 or 1-213 of SEQ ID NO:2.
Essentially homogeneous purified elk-L protein, wherein said elk-L protein is a fragment of the amino acid sequence presented as amino acids 1-322 of SEQ ID NO:2, wherein said elk-L protein binds elk.
A purified elk-L protein which binds elk, wherein the mature form of said elk-L is characterized by:
- a) a calculated molecular weight of about 35,180 daltons;
- b) a pI of about 9.006;
- and
- c) the N-terminal amino acid sequence:
- Ala-Thr-Pro-Leu-Ala-Lys-Asn-Leu-Glu-Pro-Val-Ser-.
A purified elk-L protein that is a naturally occurring variant of the elk-L presented as amino acids 1-322 of SEQ ID NO:2, wherein:
- a) the elk-L variant binds elk;
- b) the elk-L variant is encoded by a DNA which hybridizes under severely stringent conditions to a DNA consisting of nucleotides 1 to 1041 of SEQ ID NO:1;
- and
- c) the amino acid sequence of the elk-L variant is at least 80% identical to a sequence selected from the group consisting of amino acids 1-322 of SEQ ID NO:2 and amino acids 1-213 of SEQ ID NO:2.
A purified elk-L protein capable of binding elk, wherein said elk-L protein comprises an amino acid sequence that is at least 80% identical to a sequence selected from the group consisting of amino acids 1-322 of SEQ ID NO:2 and amino acids 1-213 of SEQ ID NO:
- 2.
An elk-L protein encoded by the elk-L cDNA insert in the recombinant vector deposited in strain ATCC 69085.
Dependencies are inferred from the claim text.