Enhancement of lipid cationic transfections in the presence of serum
AssigneeLife Technologies Corporation
InventorsPoJen Shih, Pamela Hawley-Nelson, Joel A. Jessee
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Abstract
A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition in the presence of a polycationic compound and serum. The polycationic compound is preferably Polybrene.TM., and the lipid aggregate preferably comprises liposomes of nucleic acid and LipofectAMINE.TM..
Claims11
In a method of transfecting an animal cell in the presence of serum, comprising contacting said cell with a lipid aggregate comprising nucleic acid and a cationic lipid, wherein the improvement comprises:
- contacting said cell with said lipid aggregate in the presence of a polycationic compound, thereby transfecting said animal cell with said nucleic acid.
In a method of transfecting an animal cell in the presence of serum, comprising contacting said cell with a lipid aggregate comprising nucleic acid and a cationic lipid, wherein the improvement comprises:
- either (a) first contacting said cell with a polycationic compound to form a complex of said cell with said polycationic compound followed by contacting said cell complex with said lipid aggregate;
- or (b) first contacting said lipid aggregate with said polycation compound to form a mixture followed by contacting said cell with said mixture, thereby transfecting said animal cell with said nucleic acid.
Dependencies are inferred from the claim text.