Refunctionalized oxyfluorinated surfaces
AssigneeThe Research Foundation of State University of New York
InventorsJoseph A. Gardella, Jr., Terrence G. Vargo
Life of this patent
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Abstract
Permanently substituted oxyfluorinated surfaces can be formed on non-fluorinated substrates having a fluorinated surface or fluorocarbon coating applied by gas phase surface fluorination or plasma deposition. The oxyfluorinated surfaces can be refunctionalized by bonding organosilanes, isothiocyanate-containing fluorescent compounds and proteins, such as enzymes, antibodies and peptides directly to such surfaces. Surfaces refunctionalized with such protein based groups are useful in the fabrication of biological sensors, devices for separation of cell lines, filtration applications for selective binding of antigens. Masking techniques can be employed in forming a predetermined pattern of covered and exposed surfaces, for example, prior to oxyfluorination.
Claims19
An oxyfluorinated substrate comprising a non-fluorinated material selected from the group consisting of polymeric, ceramic and metallic materials, the surface of said polymeric material modified with (a) molecularly bonded fluorine atoms, or (b) a fluorocarbon film, the surface of said ceramic and metallic materials modified with a fluorocarbon film, the modified surfaces having up to 98 percent of their fluorine atoms to depths from about 10 to about 100 .ANG. permanently substituted with hydrogen and oxygen or oxygen-containing groups to form substrates with oxyfluorinated surfaces of which from about 3 to about 30 percent of the substituted fluorine is replaced with oxygen or oxygen-containing groups and from about 70 to about 97 percent of the fluorine is replaced with hydrogen atoms, the morphological and hydrophobic properties of the oxyfluorinated surfaces remaining substantially unchanged from that of the unmodified surfaces having said fluorine atoms and fluorocarbon films while wettability with respect to low surface tension liquids and surface free energy (.gamma..sub.s) as determined through critical surface tension (.gamma..sub.c) are increased.
A refunctionalized oxyfluorinated substrate, which comprises a non-fluorinated material selected from the group consisting of polymeric, ceramic and metallic materials, the surface of said polymeric material modified with (a) molecularly bonded fluorine atoms, or (b) a fluorinated film, the surface of said ceramic and metallic materials modified with a fluorocarbon film, the modified surfaces having up to 98 percent of their fluorine atoms to depths from about 10 to about 100 .ANG. permanently substituted with hydrogen and oxygen or oxygen-containing groups to form substrates with oxyfluorinated surfaces of which from about 3 to about 30 percent of the substituted fluorine is replaced with oxygen or oxygen-containing groups and from about 70 to about 97 percent of the fluorine is replaced with hydrogen atoms, said oxyfluorinated surfaces being refunctionalized where from about 3 to about 100 percent of said oxygen or oxygen-containing groups have molecularly bonded thereto a member selected from the group consisting of an organosilane, an isothiocyanate-containing fluorescent compound and a protein.
A method of making a refunctionalized oxyfluorinated substrate which comprises the steps of:
- a) providing a non-fluorinated base material selected from the group consisting of a polymeric, ceramic and metallic materials;
- b) modifying the surface of:
- (i) said polymeric base material by a step selected from the group consisting of fluorination and coating with a fluorocarbon film;
- (ii) said ceramic and metallic material by the step of coating with a fluorocarbon film;
- c) oxyfluorinating the modified surface of (b) with a gas/vapor plasma mixture comprising hydrogen and at least one other member selected from the group consisting of water, methanol and formaldehyde while exposing said substrate to at least one radio frequency glow discharge under vacuum for a sufficient period to substitute at least a portion of the fluorine atoms on the substrate to a depth from about 10 to 100 .ANG. with covalently bonded hydrogen and oxygen atoms or oxygen-containing groups, and
- d) refunctionalizing said oxyfluoroinated surface of (c) by reacting at least a portion of said oxygen atoms or oxygen-containing groups with a member selected from the group consisting of an organosilane, an isothiocyanate-containing fluorescent compound and a protein.
A method of making a patterned refunctionalized oxyfluorinated substrate, which comprises the steps of:
- a) providing a substrate comprising a fluorinated material;
- b) masking portions of the substrate of step (a) to form a predetermined pattern of covered and exposed surfaces;
- c) oxyfluorinating the masked surface of the substrate of step (b) by contacting with a gas/vapor plasma mixture comprising hydrogen and at least one other member selected from the group consisting of water, methanol and formaldehyde while exposing said substrate to at least one radio frequency glow discharge under vacuum for a sufficient period to substitute at least a portion of the fluorine atoms on the exposed surface to a depth from about 10 to 100 .ANG. with covalently bonded hydrogen and oxygen atoms or oxygen-containing groups;
- d) refunctionalizing the substrate of step (c) by reacting at least a portion of said oxygen atoms or oxygen-containing groups on the exposed portions of the substrate with a member selected from the group consisting of an organosilane, an isothiocyanate-containing fluorescent compound and a protein.
Dependencies are inferred from the claim text.