Multivalent dextran reagent for use in precipitation tests
AssigneeBoehringer Mannheim GmbH
InventorsJohann Karl, Josef Maier
Life of this patent
Estimating the term…
Abstract
The present invention concerns a multivalent dextran reagent for use in a precipitation test for the determination of a specifically bindable substance comprising dextran to which several molecules of a receptor R.sub.1 which is capable of specific binding to the substance to be determined or of the specifically bindable substance or of an analogue of this substance are bound or can be bound.
Claims10
A nephelometric or turbidimetric method for determining a specifically bindable substance, which substance is bivalent or polyvalent, comprising
- providing a reaction mixture by reacting a test sample suspected for containing the specifically bindable substance with a precipitation reagent comprising a solution of multivalent dextran reagent comprising soluble dextran of molecular weight 10,000 to about 2 million, having a plurality of receptors R1 covalently bound thereto, where said R1 specifically binds to any of the specifically bindable substance in the test sample to form a precipitate which remains in solution in said reaction mixture,
- measuring the extent of precipitation in said reaction mixture by nephelometric or turbidimetric measurement means to determine the amount of the specifically bindable substance present in the test sample.
A nephelometric or turbidimetric method for determining a specifically bindable substance which substance is monovalent, comprising
- providing a reaction mixture by reacting a test sample suspected for containing the specifically bindable substance with a precipitation reagent comprising a solution of multivalent dextran reagent comprising soluble dextran of molecular weight 10,000 to about 2 million, having a plurality of molecules of the specifically bindable substance or an analogue of the specifically bindable substance covalently bound thereto, further reacting a receptor R2 which is at least bivalent, wherein said R2 specifically binds any specifically bindable substance in the test sample and the analogue to form a precipitate which remains in solution in said reaction mixture,
- measuring the extent of precipitation in said reaction mixture by nephelometric or turbidimetric measurement means to determine the amount of the specifically bindable substance present in the test sample.
Dependencies are inferred from the claim text.