Stable diagnostic reagent
AssigneeBoehringer Mannheim GmbH
InventorJoseph E. Bateson
Life of this patent
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Abstract
The inclusion of succinic acid or a salt thereof to stabilize compositions that include glucose oxidase and/or ferricyanide. Typically, the succinic acid or succinic acid salt is included in a diagnostic reagent utilized in test strips or test solutions useful for the analysis of glucose from a fluid sample.
Claims20
A process for stabilizing a diagnostic reagent composition comprising glucose oxidase, the process comprising the step of
- adding succinic acid or a salt thereof to the composition in an amount effective to stabilize the composition.
A process for stabilizing a diagnostic reagent composition comprising ferricyanide, the process comprising the step of
- adding succinic acid or a salt thereof to the composition in an amount effective to stabilize the composition.
A process for stabilizing a diagnostic reagent composition comprising glucose oxidase and ferricyanide, the process comprising the step of
- adding succinic acid or a salt thereof to the composition in an amount effective to stabilize the composition.
A diagnostic reagent composition, comprising
- glucose oxidase and ferricyanide in an aqueous medium, or in a dried matrix, the ferricyanide being present in an amount effective to function as a redox mediator in the catalytic oxidation of glucose;
- and
- succinic acid or a salt thereof in a concentration of about 18.5 millimolar to about 55 millimolar to stabilize the composition.
A method for forming a stabilized rehydratable diagnostic reagent composition comprising glucose oxidase and ferricyanide, the method comprising the steps of
- forming an aqueous solution comprising glucose oxidase, ferricyanide in an amount sufficient to function as a redox mediator in the catalytic oxidation of glucose by the glucose oxidase, a surfactant, and succinic acid or a salt thereof in an amount sufficient to stabilize the composition, and
- drying the solution to remove at least about 90% by weight water to form a stabilized dry reagent composition.
Dependencies are inferred from the claim text.