Method of reducing interference in a fluorescent assay
AssigneeCIS Bio International
InventorsGerard Mathis, Christophe DuMont, Etienne J. Jolu
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Abstract
The invention relates to a method of reducing interference in a fluorescent assay of an analyte, which comprises adding fluoride ions to the measuring medium, and to its use in a fluorescent method of detecting and/or determining an analyte in a medium which may contain it.
Claims20
A method of reducing interference in a fluorescent assay of an analyte using at least one fluorescent compound as the tracer, which comprises adding fluoride ions to the measuring medium.
A method for reducing interference in the measuring medium during a fluorescent method of detecting and/or determining an analyte in a medium which may contain it comprising adding fluoride ions to the measuring medium.
A method for reducing interference in the measuring medium during a fluorescent method of detecting and/or determining an analyte in a medium which may contain it, consisting of adding fluoride ions to the measuring medium, comprising:
- a) adding, to said medium containing the analyte in question, a first reagent consisting of at least one receptor specific for said analyte, coupled with a fluorescent donor compound,
- b) adding a second reagent consisting of one or more other receptors specific for said analyte, said second reagent being coupled with a fluorescent acceptor compound,
- c) incubating said medium either after the addition of each reagent or after the addition of both reagents,
- d) exciting the resulting medium at the excitation wavelength of the fluorescent donor compound by means of a light source, and
- e) measuring the signal emitted by the fluorescent acceptor compound.
Dependencies are inferred from the claim text.