Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
AssigneeMonsanto Company
InventorsGerard F. Barry, Ganesh M. Kishore, Stephen R. Padgette, William C. Stallings
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Abstract
Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.
Claims8
In a method for the transformation and regeneration of transgenic plants, the improvement which comprises the use of a glyphosate-resistance marker gene comprising:
- i) a promoter which functions in plant cells to cause the production of an RNA sequence,
- ii) a structural DNA sequence that causes the production of an RNA sequence which encodes an EPSPS enzyme having the sequence domains:
- -R-X.sub.1 -H-X.sub.2 -E- (SEQ ID NO:37), in which
- X.sub.1 is G, S, T, C, Y, N, Q, D or E;
- X.sub.2 is S or T;
- and
- -G-D-K-X.sub.3 - (SEQ ID NO:38), in which
- X.sub.3 is S or T;
- and
- -S-A-Q-X.sub.4 -K- (SEQ ID NO:39), in which
- X.sub.4 is A, R, N, D, C, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y or V;
- and
- -N-X.sub.5 -T-R- (SEQ ID NO:40), in which
- X.sub.5 is A, R, N, D, C, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y or V;
- and
- iii) a 3' non-translated DNA sequence which functions in plant cells to cause the addition of a stretch of polyadenyl nucleotides to the 3' end of the RNA sequence;
- where the promoter is heterologous with respect to the structural DNA sequence and adapted to cause sufficient expression of the polypeptide to render a plant cell transformed with the DNA molecule tolerance to a toxic level of glyphosate.
Dependencies are inferred from the claim text.