2'deoxy-2'-alkylnucleotide containing nucleic acid
AssigneeRibozyme Pharmaceuticals, Inc.
InventorsNassim Usman, Alexander Karpeisky, Leonid Beigelman, Anil Modak
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Abstract
2'-deoxy-2'-alkylnucleotides useful for stabilizing enzymatic nucleic acid molecules and antisense molecules.
Claims5
Oligonucleotide comprising one or more 2'-deoxy-2'-alkyl-nucleotides.
Enzymatic nucleic acid Capable of cleaving single-stranded RNA or DNA comprising a 2'-deoxy-2'-alkylnucleotide.
Method for producing an enzymatic nucleic acid molecule having enzymatic activity capable of cleaving a single-stranded RNA or DNA molecule, comprising the step of forming said enzymatic molecule with at least one nucleotide having at its 2'-position an alkyl group.
2'-deoxy-2'-alkylnucleotide triphosphate.
Method for synthesis of a 2'-C-allyl derivative of a nucleoside from a 5'-O-dimethoxytrityl-3 '-O-t-bulydimethylsilyl-base comprising the steps of:
- (a) Phenoxythiocarbonylation of 5'O-dimethoxytrityl-3'-O-t-bulydimethylsilyl-base to yield a thioester, replacing a 2' hydroxyl group with a phenoxythiocarbonyl groups, and
- (b) Heck allylation of said thioester to form a 2'-C-allyl derivative in which said 2'-phenoxythiocarbonyl group is replaced with a 2'-allyl group to yield said 2'-C-allyl derivative.
Dependencies are inferred from the claim text.