Bone-related sulfatase-like protein and process for its production
AssigneeHoechst Japan Limited
InventorsSunao Takeshita, Toshimi Ito, Yoko Otawara-Hamamoto, Egon Amann
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Abstract
A bone-related protein named OSF-8 which is obtained from bone tissue of a mammal including mouse or human, and a process for its production. This protein is a novel naturally occurring mammal protein which belongs to a group of sulfatases. OSF-8 degrades the sulfate groups of the sugar chains of proteoglycan, which makes up the cartilage matrix, during the replacement of cartilage into bone tissue at the stage of osteogenesis. OSF-8 can be used as an agent for treating bone metabolic diseases, and its high organ specificity for bones enables its use as a diagnostic reagent for bone metabolic diseases.
Claims3
A process for the production of a recombinant mammalian osteocyte specific factor OSF-8 protein comprising the steps of:
- (a) obtaining a population of cells containing a heterologous DNA composed of the following DNA sequences:
- (i) a sequence that can function in the cells to control transcription and translation, and
- (ii) a DNA sequence joined downstream of said controlling sequence to code for said recombinant OSF-8 protein, and
- (b) culturing said population of cells under conditions which permit the production of said recombinant protein;
- and wherein said OSF-8 protein comprises mouse OSF-8 having an amino acid sequence at the 19th to 556th positions in Sequence ID No. 2 of the Sequence Listing.
Dependencies are inferred from the claim text.