Aedes aegypti densovirus expression system

An expression vector comprising the 5' and 3'-termini of AeDNV genomic DNA flanking at least one expression cassette comprising an AeDNV promoter and a cloning site wherein said cloning site is operably linked to said promoter.

A method of producing a recombinant protein comprising:

• transfecting an insect cell with an expression vector comprising the 5' and 3' termini of AeDNV genomic DNA flanking at least one expression cassette comprising an AeDNV promoter operably linked to the coding sequence for said recombinant protein;
• culturing said cell under conditions whereby said protein is expressed;
• and recovering said protein.

A method of producing a recombinant protein comprising:

• co-transfecting an insect cell with a first expression vector comprising the 5' and 3'-termini of AeDNV genomic DNA flanking at least one expression cassette comprising an AeDNV promoter operably linked to the coding sequence of said protein, and a second expression vector comprising the AeDNV p0.5 promoter and the left ORF;
• culturing said cell under conditions whereby said protein, NS1 and NS2 are expressed;
• and recovering said protein.

A method of producing a recombinant protein comprising:

• transfecting an insect cell with an expression vector comprising the 5' and 3'-termini of AeDNV genomic DNA flanking at least one expression cassette comprising an AeDNV promoter operably linked to the coding sequence for said protein and further flanking an expression cassette comprising the AeDNV p0.5 promoter and the left ORF of AeDNV;
• culturing said cell under conditions whereby said protein, NS1 and NS2 are expressed;
• and recovering said protein.

A method of producing a recombinant protein comprising:

• transfecting an insect cell that stably expresses NS1 and NS2 with an expression vector comprising the 5' and 3'-termini of AeDNV genomic DNA flanking at least one expression cassette comprising an AeDNV promoter operably linked to the coding sequence of said protein;
• culturing said cell under conditions whereby said protein is expressed;
• and recovering said protein.

An expression vector comprising the 5' and 3'-termini of AeDNV genomic DNA flanking a first expression cassette comprising at least one of the p0.5 or p7 promoters of AeDNV operably linked to a first heterologous gene and a second expression cassette comprising the p61 promoter of AeDNV operably linked to a second heterologous gene.

An expression vector for targeting a recombinant protein to the cell nucleus comprising the 5' and 3'-termini of AeDNV genomic DNA flanking an expression cassette comprising the p61 promoter and the coding region of about the first thirty eight amino acids of VP operably linked to the coding region of said recombinant protein.