Yeast strains used to identify inhibitors of dibasic amino acid processing endoproteases
AssigneeThe Regents of the University of Colorado
InventorAlex Franzusoff
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Abstract
The present invention relates to novel yeast strains which produce a heterologous precursor protein having a dibasic amino acid processing site which can be processed into at least one cleavage protein by a dibasic amino acid processing endoprotease. Such novel yeast strains are useful for identifying compounds capable of inhibiting infectious agents, such as viruses, that depend upon dibasic amino acid processing endoprotease cleavage for effective propagation and/or infectivity.
Claims17
A yeast strain which produces a heterologous dibasic amino acid processing endoprotease and a heterologous precursor protein of an infectious agent, said precursor protein having a dibasic amino acid processing site, wherein said yeast strain processes said precursor protein into at least one cleavage protein.
A Kex2 endoprotease-deficient yeast strain which produces a heterologous precursor protein having a dibasic amino acid processing site, wherein said yeast strain does not process said precursor protein into at least one cleavage protein.
A yeast strain which produces a heterologous dibasic amino acid processing endoprotease, wherein said endoprotease processes a precursor protein having a dibasic amino acid cleavage site into at least one cleavage protein.
Dependencies are inferred from the claim text.