Use of an anticoagulant as a diagnostic agent
AssigneeBoehringer Ingelheim International GmbH
InventorChristiaan Reutelingsperger
Life of this patent
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Abstract
Detectably labelled annexines and compositions thereof are disclosed. Also disclosed are methods for diagnosing a disruption or activation of the hemostatic system or a prothrombotic state in an individual suspected of having a hemostatic disorder, by contacting the blood of said individual with an annexine, and detecting whether an annexine-platelet complex is formed.
Claims27
A method for diagnosing a prothrombic state in an individual comprising:
- (a) contacting the blood of said individual with an annexine in the presence of calcium;
- (b) detecting whether an annexine-platelet complex is formed;
- and
- (c) correlating the forenation of an annexine-platelet complex with a prothrombic state.
A method for distinguishing between phosphatidyl serine and phosphatidyl choline, comprising contacting a sample suspected of containing phosphatidyl serine or phosphatidyl choline with an annexine to allow formation of an annexine/phosphatidyl serine complex, and detecting whether a complex has formed, thereby distinguishing between phosphatidyl serine and phosphatidyl choline.
A method for detecting the point of the activation of the hemostatic system, comprising:
- (a) introducing a detectably labeled annexine into the blood of an individual suspected of having a hemostatic disorder;
- (b) permitting the binding of said annexine to phosphatidyl serine present on the surface of activated platelets;
- (c) determining the distribution of said detectably labeled annexine bound to the surface of activated platelets;
- and
- (d) correlating the distribution of said detectably labeled annexine with the point of activation of the hemostatic system.
A method for detecting the prothrombotic state of an individual suspected of having a hemostatic disorder, comprising:
- (a) the blood of said individual is contacted extracorporally with a detectably labeled annexine;
- (b) the presence of said delectably labeled annexine bound to the surface of weakly activated platelets is detected;
- and
- (c) the presence of annexine bound to the surface of weakly activated platelets is correlated with a prothrombotic state.
A method for quantitating the amount of phosphatidyl serine on a phospholipid surface, comprising:
- (a) contacting a detectably labeled annexine with a phospholipid surface to be assayed in the presence of calcium;
- (b) determining the extent of binding of said annexine to said phospholipid surface;
- and
- (c) comparing the extent of binding to a standard curve to obtain the concentration of phosphatidyl serine on said phospholipid surface.
A kit for the diagnostic detection of the prothrombic state or a thrombus, comprising
- a carrier means being compartmentalized to receive container means;
- a first container means which contains a detectably labeled annexine;
- and
- (c) a second container means which contains a standard concentration of phosphatidyl serine.
Dependencies are inferred from the claim text.