Mammalian expression vectors
AssigneeResearch & Diagnostics Systems, Inc.
InventorsJohn M. Smith, John E. Humphrey, Monica L. Tsang, James A. Weatherbee
Life of this patent
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Abstract
A vector system that allows the rapid and effective screening of recombinant constructs. The vector system includes a marker protein useful for identifying transfected cell lines, wherein the promoter used to express the marker protein has be substantially weakened in comparison to its corresponding wild type form.
Claims29
A vector useful for selection and expression of a cDNA gene sequence in a mammalian system, the vector comprising;
- a) a cDNA structural gene sequence encoding a protein product of choice;
- b) a cDNA promoter sequence controlling the cDNA sequence;
- c) a native marker protein sequence encoding a detectable protein product;
- and
- d) a marker promoter sequence controlling the marker protein sequence;
- wherein the cDNA structural gene and marker promoter sequences are cotransformed, and the marker sequence promoter is mutated so as to exhibit an activity level substantially below that of its corresponding wild type.
A method of making a vector useful for selection and expression of a cDNA gene sequence in a mammalian system, the method comprising the steps of providing
- a) a cDNA structural gene sequence encoding a protein product of choice;
- b) a cDNA promoter sequence controlling the cDNA structural gene sequence;
- c) a native marker protein sequence encoding a detectable protein product;
- and
- d) a marker promoter sequence controlling the marker protein sequence;
- and
- using each sequence to construct a vector wherein the cDNA structural gene and marker promoter sequences are cotransformed, and the marker sequence promoter is mutated so as to exhibit an activity level substantially below that of its corresponding wild type.
Dependencies are inferred from the claim text.