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US 5,626,848 AGranted
Reduced-cloud-point clarified neem oil and methods of producing
AssigneeThermo Trilogy Corporation
InventorsDeborah H. Barnette, James F. Walter
Life of this patent
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Abstract
A clarified neem oil having a cloud point below 13.degree. C. exhibits reduced phytotoxicity. Treatment of crude neem oil with a dilute aqueous basic solution or with an enzyme having lipid-degrading activity yields a clarified neem oil having acceptably low phytotoxicity. The methods are advantageous because they reduce or eliminate the waste stream of waxy by-product generated using existing methods for clarifying crude neem oil.
Claims3
A method for preparing a neem oil having an acceptably low foliar phytotoxicity, the method comprising the steps of:
- treating a crude neem oil preparation comprising lipid material with an amount of an enzyme having a lipid-degrading activity, at a temperature at which the enzyme retains the lipid-degrading activity to degrade a percentage of the lipid material until a clarified neem oil is formed;
- inactivating the enzyme;
- and
- determining the foliar phytotoxicity of the enzyme-treated neem oil,
- wherein the foliar phytotoxicity of the clarified neem oil is lower than the foliar phytotoxicity of the crude neem oil.
2 more elements
Dependencies are inferred from the claim text.