Monoclonal antibody against ricin A chain
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsPaul V. Lemley, D. Craig Wright
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Abstract
Monoclonal antibodies against the A chain of ricin have been found to be ective in protecting mammals from morbidity arising from exposure to ricin toxin. The neutralizing action of the antibodies does not appear to be mediated by complement or by immunoprecipitation. The antibodies of the invention are characterized as of isotype IgG1 having the binding characteristics which include: a) binding specifically to the neutralizing epitope of the ricin A chain and b) providing in vitro protection of at least 95% of EL-4 cells from 100 .eta.g/mL ricin challenge when said antibody is present in the tissue culture at a level of at least 1000 .eta.g/mL.
Claims10
A monoclonal antibody of isotype IgGl having the binding characteristics of the antibody produced by the hybridoma which is deposited in the American Type Culture Collection and assigned the ATCC accession number HB-12054 said characteristics include:
- a) binding specifically to the neutralizing epitope of the ricin A chain;
- and
- b) providing in vitro protection of at least 95% of EL-4 cells from 100 ng/mL ricin challenge when said antibody is present in the tissue culture at a level of at least 1000 ng/mL.
Dependencies are inferred from the claim text.