Methods for the separation and detection of nitrosothiols
AssigneeAmerican Health Foundation
InventorJohn P. Richie
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Abstract
A method for detecting a nitrosothiol in an eluant from a chromatographic process including passing the eluant through a series of electrodes comprising an upstream working electrode and a downstream working electrode. The upstream working electrode is maintained at a potential adequate to reduce the nitrosothiol to a corresponding thiol. The downstream electrode is maintained at a potential to detect the corresponding thiol.
Claims21
A method for detecting a nitrosothiol in an eluant comprising a) passing the eluant through a series of electrodes comprising an upstream working electrode and a downstream working electrode, the upstream working electrode being maintained at a potential adequate to reduce said nitrosothiol to a corresponding thiol, said downstream electrode being maintained at a potential to detect said corresponding thiol, and b) detecting said correspond thiol.
A method for reducing a nitrosothiol (RSNO) to a corresponding thiol (RSH), said method comprising passing a solvent containing said nitrosothiol through an electrochemical cell comprising a working electrode maintained at a potential adequate to reduce said nitrosothiol to said corresponding thiol.
A method of liquid chromatographic analysis to separate and detect nitrosothiols from other components in a sample solution, said method comprising
- a) flowing the solution through chromatographic separation means to separate the nitrosothiols from the other components upon elution thereof;
- b) passing an effluent from said chromatographic means through a first flow-through cell wherein an electric potential is applied to an upstream working electrode to reduce the nitrosothiols to their corresponding thiols;
- and
- c) detecting the corresponding thiols formed in step (b) by passing the effluent through a second flow-through cell wherein an electrical potential is applied to a downstream working electrode.
Dependencies are inferred from the claim text.