Electrophoresis slab gel enclosure for gradient gels
AssigneeBio-Rad Laboratories, Inc.
InventorDaniel Y. Chu
Life of this patent
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Abstract
Gradient gels are formed in a slab gel enclosure by introducing gel solution through a channel in one of the two or more spacers which set the thickness of the gel. The channel opens into the gel space through an aperture in the inner longitudinal edge of the spacer at approximately mid-length along the edge, and filling of the gel space with the solution is performed with the enclosure rotated onto its side. In certain embodiments of the invention, the opposing spacer contains an aperture as well, plus a channel leading from the aperture to the exterior of the enclosure, for venting of air from the gel space as the space is being filled with the gel-forming liquid.
Claims6
An enclosure for a slab gel for electrophoretic separations, comprising:
- first and second plates, each with four edges defined as two opposing side edges, a top edge and a bottom edge;
- first and second spacer strips for placement between said plates to define an open space for said slab gel;
- a first aperture in a longitudinal edge of said first spacer strip at a location displaced from either end thereof, a second aperture in said first spacer strip at a location other than said longitudinal edge and closer to one end of said first spacer strip, means for communicating said second aperture with a supply of gel-forming liquid, and a channel in said first spacer strip communicating said first aperture with said second aperture;
- and
- clamping means for clamping said first and second plates together with said first and second spacer strips in between.
Dependencies are inferred from the claim text.