Process for culturing hepatocytes for formation of spheroids
AssigneeSeikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation)
InventorsToshikazu Yada, Norio Koide, Koji Kimata
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Abstract
Hepatocytes spheroids can be formed by culturing hepatocytes in a culture vessel using a lipid-bound glycosaminoglycan as a culture substrate. Floating spheroids of hepatocytes can be obtained efficiently, which are capable of maintaining liver-specific functions and of keeping the spheroid form stably for a prolonged period of time.
Claims3
A process for forming spheroids of hepatocytes which comprises culturing hepatocytes with a lipid-bound glycosaminoglycan as a culture substrate, wherein said lipid-bound glycosaminoglycan is phosphatidyl ethanolamine diacyl bound chondroitin sulfate.
A process for forming spheroids of hepatocytes which comprises culturing hepatocytes with a lipid-bound glycosaminoglycan as a culture substrate, wherein said lipid-bound glycosaminoglycan is phosphatidyl ethanolamine dipalmitoyl bound chondroitin sulfate.
A process for forming spheroids of hepatocytes which comprises culturing hepatocytes with a lipid-bound glycosaminoglycan as a culture substrate, wherein said lipid-bound glycosaminoglycan has an ICs.sub.50 for cell adhesion inhibition activity of 1.49 .mu.g/ml or less.
Dependencies are inferred from the claim text.