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US 5,622,857 AGranted
High performance cell culture bioreactor and method
AssigneeGenespan Corporation
InventorRandal A. Goffe
Life of this patent
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Abstract
A high performance hollow fiber bioreactor having concentric hollow fiber bundles: a central hollow fiber bundle supplies media, and an outer array supplies oxygen needed for cell culture. Useful to expand therapeutic cells such as stem cells ex vivo, and as an extracorporeal device such as an artificial liver.
Claims46
A bioreactor comprising:
- a housing comprising an inner wall defining a substantially tubular cell culture chamber, first and second ends defining a longitudinal axis through said chamber, and a port providing access to said chamber,
- a central bundle of porous hollow fibers disposed around the longitudinal axis within said chamber,
- an annular bundle of gas permeable hollow fibers disposed concentric to and surrounding said central bundle,
- means disposed at said first and second ends for passing nutrient media through said porous hollow fibers, and
- means disposed at said first and second ends for passing an oxygen containing gas through said gas permeable hollow fibers.
2 more elements
The method of claim30, wherein said harvesting step comprises introducing a proteolytic enzyme into the chamber.
A bioreactor comprising:
- a housing comprising an inner wall defining a substantially tubular cell culture chamber, the chamber having first and second ends, a longitudinal axis, and a port providing access to said chamber;
- a central bundle of porous hollow fibers surrounding the longitudinal axis of said chamber, each of the fibers in fluid communication with a common axially-extending liquid inlet port;
- and
- an annular bundle of gas permeable hollow fibers disposed concentric with, and surrounding, said central bundle, each of the gas permeable fibers of the annular bundle having an outlet end and an inlet end, the outlet ends of the gas permeable fibers in fluid communication with a common annular space surrounding the axially-extending liquid inlet port, a gas outlet port in fluid communication with the common annular space.
1 more element
Dependencies are inferred from the claim text.