Method for increasing the omega-hydroxylase activity in Candida tropicalis
AssigneeHenkel Corporation
InventorsStephen Picataggio, Tracy Rohrer, L. Dudley Eirich
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Abstract
The omega-hydroxylase activity in the yeast Candida tropicalis is increased by amplification of the P450ALK and P450RED genes. The specific productivity (grams of dicarboxylic acid/liter/hr) of substantially pure alpha, omega-dicarboxylic acids is increased by culturing C. tropicalis strains having amplified P450ALK and P450RED genes.
Claims18
A process for producing alpha, omega-dicarboxylic acids comprising culturing a C. tropicalis strain having all four POX genes disrupted and having an increased copy number of at least one P450ALK gene, at least one P450RED gene, or a combination of said genes in a culture medium containing a nitrogen source, an organic substrate and a cosubstrate.
A process for producing alpha, omega-dicarboxylic acids comprising culturing a C. tropicalis strain having all four POX genes disrupted and having an increased copy number of a P450RED gene in a culture medium containing a nitrogen source, an organic substrate and a cosubstrate.
Dependencies are inferred from the claim text.