Immunoassay diagnostic kit
AssigneeAmpcor, Inc.
InventorsMartin Gould, Sudhakar Vulimiri
Life of this patent
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Abstract
An immunoassay process is provided for the detection of a target antigen in a fluid sample where an admixture of the target antigen and a labeled capture reagent against the target antigen is contacted by a solid polymeric carrier having bound to a portion of its surface a capture reagent against the target antigen and visual determination of a change in color of the bound labeled reaction product on the surface of the solid carrier member serves to readily detect the presence of the target antigen.
Claims20
An immunoassay process for the detection of a target immunologically active agent in a liquid sample comprising:
- a) contacting said liquid sample containing said target immunologically active agent to be assayed with a labeled capture reagent against said target immunologically active agent, and with a controlled effective amount of a bound capture reagent against said target immunologically active agent bound to a solid carrier member over only a portion thereof in a controlled substantially specific array and wherein the remaining portion of said solid carrier member having been treated with animal serum and with a solution of a casein protein in an effective amount is substantially blocked against bonding to said labeled capture reagent and said target immunologically active agent;
- and
- b) detecting the presence of said target immunologically active agent by determining the label bound to said bound capture reagent on said solid carrier member as an indication of the presence of the target immunologically active agent in said fluid sample.
An immunoassay process for the detection of a target immunologically active agent in a liquid sample consisting essentially of admixing said liquid sample containing said target immunologically active agent with a labeled capture reagent against said target immunologically reactive agent, contacting said admixture with a controlled effective amount of a bound capture reagent against said target immunologically active agent, said bound capture reagent being applied to and bound to a solid carrier member by jet-type atomizer means over only one surface thereof in a controlled substantially specific array and wherein the remaining portion of said solid carrier member is substantially blocked against bonding to said labeled capture reagent and said target immunologically reactive agent, and then contacting said bound capture reagent without washing the same with a color forming solution selected for detecting the presence of the target immunologically active agent by determining by visualization an indication of the presence of label bound to said bound capture reagent on said carrier member.
Dependencies are inferred from the claim text.