Modular assembly of antibody genes, antibodies prepared thereby and use
AssigneeXoma Corporation
InventorsRandy R. Robinson, Alvin Y. Liu, Arnold H. Horwitz, Marc Better, Randolph Wall, Shau-Ping Lei, Gary L. Wilcox
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Abstract
The invention relates to the secretion of heavy chain immunoglobulin fragments and light chain immunoglobulins from prokaryotic hosts using a prokaryotic secretion signal peptide wherein the heavy chain fragments and light chains are capable of associating to form an antigen binding antibody fragment.
Claims45
A secretable immunoglobulin heavy chain fragment comprising an entire variable region directly linked to a prokaryotic secretion signal peptide, wherein said secretable heavy chain fragment is capable of being secreted from a prokaryotic organism and of providing an immunoglobulin heavy chain fragment that is capable of associating with an immunoglobulin light chain, or with an immunoglobulin light chain fragment that comprises an entire variable region, and, when so associated, of binding antigen.
A secretable immunoglobulin light chain, or secretable light chain fragment comprising an entire variable region, directly linked to a prokaryotic secretion signal peptide, wherein said secretable light chain or secretable light chain fragment is capable of being secreted from a prokaryotic organism and of providing an immunoglobulin light chain or light chain fragment that is capable of associating with an immunoglobulin heavy chain fragment that comprises an entire variable region, and, when so associated, of binding antigen.
A method of producing an immunoglobulin heavy chain fragment comprising an entire variable region, said method comprising the steps of:
- 1) expressing, in a prokaryotic organism, a polynucleotide sequence encoding a secretable immunoglobulin heavy chain fragment that comprises an entire variable region directly linked to a prokaryotic secretion signal peptide,
- 2) secreting said secretable immunoglobulin heavy chain fragment from said organism so as to provide an immunoglobulin heavy chain fragment that is capable of associating with an immunoglobulin light chain, or with an immunoglobulin light chain fragment that comprises an entire variable region, and, when so associated, of binding antigen.
A method of producing an immunoglobulin light chain or light chain fragment comprising an entire variable region, said method comprising the steps of:
- 1) expressing, in a prokaryotic organism, a polynucleotide sequence encoding a secretable immunoglobulin light chain, or light chain fragment that comprises an entire variable region, directly linked to a prokaryotic secretion signal peptide,
- 2) secreting said secretable immunoglobulin light chain or light chain fragment from said organism so as to provide an immunoglobulin light chain, or light chain fragment, that is capable of associating with an immunoglobulin heavy chain fragment that comprises an entire variable region, and, when so associated, of binding antigen.
Dependencies are inferred from the claim text.