Bacterial cell tranformants for production of cis, cis-muconic acid and catechol
AssigneePurdue Research Foundation
InventorsJohn W. Frost, Karen M. Draths
Life of this patent
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Abstract
A heterologous cell transformant is provided that biocatalytically converts a carbon source to catechol and cis, cis muconic acid. The cell transformant expresses heterologous genes encoding the enzymes 3-dehydroshikimate dehydratase, protocatechuate decarboxylase, and catechol 1,2-dioxygenase.
Claims6
A bacterial cell transformed with heterologous structural genes from Klebsiella pneumoniae, which express the enzymes 3-dehydroshikimate dehydratase and protocatechuate decarboxylase, and from Acinetobacter calcoaceticus, which expresses the enzyme catechol 1,2-dioxygenase, wherein a culture of said bacterial cell biocatalytically converts 56 mM glucose to 17 mM cis, cis-muconic acid within 48 hours.
A bacterial cell transformed with heterologous structural genes from Klebsiella pneumoniae, which express the enzymes 3-dehydroshikimate dehydratase, and protocatechuate decarboxylase, wherein a culture of said bacterial cell biocatalytically converts 56 mM glucose to 16 mM catechol within 48 hours.
Dependencies are inferred from the claim text.