# US 5,616,487 A · Stabilized retrovirus compositions

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,616,487 A
- Document ID: US5616487A
- Status: Granted
- Kind: A
- Application number: 08/307,862
- Filed: 1994-09-15
- Granted: 1997-04-01
- Earliest claimed: 1994-09-15
- Assignees: Aastrom Biosciences, Inc.
- Inventors: Bernhard O. Palsson; Timothy M. Eisfeld
- Web page: https://patentgrep.com/patent/US5616487A

## CPC

- C12N 15/86: Viral vectors
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C12N 2740/13021: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- C12N 2740/13043: viral genome or elements thereof as genetic vector
- C12N 2740/13051: Methods of production or purification of viral material
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention provides a stabilized virus, which is modified using a stabilizing agent. The invention also provides a process for producing a stabilized virus by culturing viral producing cells with a stabilizing agent at a temperature below 37.degree. C. The invention further provides methods to introduce an exogenous nucleotide sequence into a cell using a stabilized virus containing the exogenous nucleotide sequence. The invention also provides methods for administering an exogenous nucleotide sequence to a subject using a stabilized virus containing the exogenous nucleotide sequence. The invention further provides a method to produce a protein by infecting a cell with a stabilized virus containing a exogenous nucleotide sequence encoding the protein and then isolating the protein produced by the infected cells.

## Claims

### Claim 1

A stabilized retrovirus composition comprising a retrovirus and an .alpha.-hydro-.omega.-hydroxypoly(oxyethylene)poly(oxypropylene)poly(oxyet hylene) block copolymer.

### Claim 2 (depends on 1)

The stabilized retrovirus composition of claim 1, wherein the .alpha.-hydro-.omega.-hydroxypoly(oxyethylene)poly(oxypropylene)poly(oxyet hylene) block copolymer is PLURONIC.RTM. F-68.

### Claim 3 (depends on 1)

A method for introducing an exogenous nucleotide sequence into a target cell in vitro or ex vivo, comprising contacting the target cell with the stabilized retrovirus composition of claim 1.

### Claim 4 (depends on 1, 2)

The stabilized retrovirus composition of claim 1 or 2 further comprising cholesterol, cod liver oil, d-.alpha.-tocopherol acetate and TWEEN 80.TM..

### Claim 5 (depends on 1, 2)

The stabilized retrovirus composition of claim 1 or 2, further comprising a target cell.

### Claim 6

A process for producing a stabilized retrovirus comprising the steps of:
a) obtaining a retrovirus producing cell;
b) contacting the retrovirus producing cell with a culture medium comprising an .alpha.-hydro-.omega.-hydroxypoly(oxyethylene)poly(oxypropylene)poly (oxyethylene) block copolymer at a temperature less than 37.degree. C.; and
c) producing retrovirus from the retrovirus producing cell.

### Claim 7 (depends on 6)

The process of claim 6, wherein the .alpha.-hydro-.omega.-hydroxypoly(oxyethylene)poly(oxypropylene)poly(oxyet hylene) block copolymer is PLURONIC.RTM. F-68.

### Claim 8 (depends on 6, 7)

The process of claim 6 or 7, wherein the culture medium further comprises cholesterol, cod liver oil, d-.alpha.-tocopherol acetate and TWEEN 80.TM..

### Claim 9 (depends on 6, 7)

The process of claim 6 or 7, wherein the temperature is less than about 30.degree. C.

### Claim 10 (depends on 6, 7)

The process of claim 6 or 7, comprising the additional step of purifying the retrovirus.

### Claim 11 (depends on 6, 7)

A stabilized virus produced by the process of claim 6 or 7.

### Claim 12 (depends on 8)

The process of claim 8 wherein the temperature is less than about 30 C.

### Claim 13 (depends on 8)

The process of claim 8, comprising the additional step of purifying the retrovirus.

### Claim 14 (depends on 8)

A stabilized retrovirus produced by the process of claim 8.

### Claim 15 (depends on 9)

The process of claim 9, wherein the temperature is about 29.degree. C.