Cloning and expression of xylanase B
AssigneeGist-Brocades N.V.
InventorsAlbert J. J. van Ooyen, Leendert H. DeGraaff, Henriette C. van den Broeck, Jacob Visser
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Abstract
Method and expression constructs are provided for the cloning of genes from fungal origin which encode polypeptides having the activity of the Aspergillus tubigensis XYL B enzyme and their overexpression in a selected microbial host cell. The present invention provides for the high level production of XYL B which may be used in a variety of industrial applications.
Claims14
A purified and isolated polynucleotide molecule which encodes a polypeptide having the xylanase activity of the Aspergillus tubigensis xylanase B enzyme wherein the nucleotide sequence of said polynucleotide molecule is selected from the group consisting of:
- a) the nucleotide sequence of FIG. 2 or SEQ ID NO:7;
- b) nucleotide sequences encoding the xylanase B polypeptide of 225 amino acids shown in FIG. 2 or SEQ ID NO:8;
- and
- c) nucleotide sequences encoding the mature xylanase B polypeptide of 188 amino acids shown at amino acid positions 38-225 in FIG. 2 or SEQ ID NO:8.
The plasmid pIM170 which has the accession number CBS-629.92 at the Centraalbureau voor Schimmelcultures (CBS).
Purified and isolated expression and transcription regulatory regions as found in the 5' non-coding sequence of the Aspergillus tubigensis xln B gene shown as nucleotides 100-263 in FIG. 2 (nucleotides 738-901 of SEQ ID NO:7).
A purified and isolated polynucleotide molecule which hybridizes under stringent conditions to the DNA molecule of FIG. 2 or SEQ ID NO:7.
Dependencies are inferred from the claim text.