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US 5,610,042 AGranted
Methods for stable transformation of wheat
AssigneeCiba-Geigy Corporation
InventorsYin-Fu Chang, James R. Wong, Andrea Itano, Stephen J. Mejza, Leslie Walker
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Abstract
The present invention is drawn to the production of fertile transformed wheat plants. The method involved subjecting wheat tissues to high velocity microprojectile bombardment, selecting for transformed cells, and regenerating stably transformed fertile plants from the transformed cells.
Claims5
A method for producing stably transformed fertile wheat plants, said method comprising:
- isolating an immature embryo from a wheat plant;
- plating said immature embryo on growth medium;
- delivering a DNA sequence of interest by high velocity microprojectile bombardment to said immature embryo up to 10 days after excision;
- treating said immature embryo in a manner sufficient to produce embryogenic callus;
- selecting for transformed cells;
- and,
- regenerating fertile transformed plants.
4 more elements
Dependencies are inferred from the claim text.