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US 5,608,152 AGranted
Seed-specific transcriptional regulation
AssigneeCalgene, Inc.
InventorsJean C. Kridl, Vic C. Knauf
Life of this patent
Estimating the term…
Abstract
Brassica plants and seeds comprising nucleic acid sequences and methods for their use are provided which afford seed-specific transcription in order to modulate or modify expression in seed particularly in embryo cells. Transcriptional initiation regions are identified and isolated from plant cells such as seed embryo and seed coat and used to prepare expression cassettes which may then be transformed into plants cells for seed specific transcription. The method finds particular use in conjunction with modifying fatty acid production in seed tissue.
Claims14
A Brassica plant comprising:
- a DNA construct comprising, in the 5' to 3' direction of transcription, a transcriptional initiation region from a gene that encodes a product preferentially expressed in a plant seed cell as compared to other plant cells, a DNA sequence of interest other than the native coding sequence of said gene, and a transcriptional termination region, wherein said gene is a napin gene, an acyl carrier protein gene or an EA9 gene.
A Brassica seed comprising:
- a DNA construct comprising, in the 5' to 3' direction of transcription, a transcriptional initiation region from a gene that encodes a product preferentially expressed in a plant seed cell as compared to other plant cells, a DNA sequence of interest other than the native coding sequence of said gene, and a transcriptional termination region, wherein said gene is a napin gene, an acyl carrier protein gene or an EA9 gene.
Dependencies are inferred from the claim text.