DNA photocleavage using texaphyrins
AssigneesPharmacyclics, Inc., Board of Trustees, Univ. of TX Sys.
InventorsDarren Magda, Jonathan L. Sessler, Brent L. Iverson, Petra I. Sansom, Meredith Wright
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Abstract
Methods of cleavage of a polymer of deoxyribonucleic acid using photosensitive texaphyrins are disclosed. A preferred method of use is the site-specific cleavage of a polymer of deoxyribonucleic acid and a preferred texaphyrin is a derivatized texaphyrin having binding specificity, in particular, a texaphyrin covalently coupled to a site-directing molecule, preferably an oligonucleotide.
Claims20
A method of light-induced cleavage of a polymer of deoxyribonucleic acid, the method comprising:
- contacting the polymer with a photosensitive texaphyrin;
- and
- exposing the photosensitive texaphyrin to light for a time sufficient to cleave the polymer.
A method for targeted intracellular light-induced cleavage of DNA, the method comprising:
- introducing into a cell a photosensitive texaphyrin coupled to an oligonucleotide having complementary binding affinity for a targeted DNA, and
- exposing the photosensitive texaphyrin to light for a time sufficient to effect cleavage of the DNA.
A method for modulating the activity of a DNA, the method comprising:
- contacting a DNA with a photosensitive texaphyrin coupled to an oligonucleotide having complementary binding affinity for said DNA, and
- exposing the photosensitive texaphyrin to light for a time sufficient to effect cleavage of the DNA.
Dependencies are inferred from the claim text.