Stroma-derived stem cell proteoglycan growth factor
AssigneeRegents of the University of Minnesota
InventorsPhilip B. McGlave, Catherine M. Verfaillie, Pankaj Gupta
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Abstract
An anionic macromolecular containing composition is disclosed that is capable of supporting conservation and differentiation of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells. The composition disclosed is a mixture of glycoproteins, more specifically proteoglycans, which are of a molecular weight greater than 200 kD.
Claims16
A synthetic proteoglycan, having a core protein and a polysaccharide portion, that is capable of promoting differentiation and maintaining the self-renewal capacity of long-term bone marrow culture initiating cells in cultured mammalian hematopoietic cells, wherein the polysaccharide portion of the synthetic proteoglycan comprises heparan sulfate, chondroitin sulfate, dermatan sulfate or a combination thereof, and the core protein comprises ovalbumin.
A method for culturing hematopoietic cells, comprising:
- culturing a population of hematopoietic cells comprising stem cells or committed progenitor cells in a culture medium comprising a synthetic proteoglycan having a core protein and a polysaccharide portion, wherein the polysaccharide portion of the synthetic proteoglycan comprises heparan sulfate, chondroitin sulfate, dermatan sulfate or combinations thereof and the core protein comprises ovalbumin, so that the ability of the stem cell population to differentiate and self-replicate is maintained or the ability of the committed progenitors to expand and differentiate is maintained.
Dependencies are inferred from the claim text.