Active programmable electronic devices for molecular biological analysis and diagnostics

An electronic device adapted to receive a solution including electrolytes and one or more charged specific binding entities, the device being adapted to affect the electrophoretic transport of the specific binding entities in the solution to selected locations on the device surface, the device comprising:

• a substrate,
• a first electrode, the electrode being supported by the substrate, the electrode being adapted to receive current,
• a permeation layer having selective diffusion properties for the passage from the solution to the electrode of the electrolytes relative to the charged specific binding entities, permitting the free transport of counter-ions through the permeation layer to the electrode, the permeation layer being disposed adjacent the first electrode, the permeation layer and the first electrode being separated by a buffer reservoir,
• an attachment layer adjacent to the permeation layer with selective binding properties for the specific binding entities, and
• a second electrode, the second electrode being electrically insulated from the first electrode prior to the application of the solution.

An electronic device adapted to receive a solution including electrolytes and one or more charged specific binding entities, the device being adapted to affect the electrophoretic transport of the specific binding entities in the solution to selected locations on the device surface, the device comprising:

• a substrate,
• a first electrode, the electrode being supported by the substrate, the electrode being adapted to receive current,
• a permeation layer aminopropyltriethoxy silane, the permeation layer being disposed adjacent the first electrode,
• an attachment layer adjacent to the permeation layer with selective binding properties for the specific binding entities, and
• a second electrode, the second electrode being electrically insulated from the first electrode prior to the application of the solution.

An electronic device adapted to receive a solution including electrolytes and one or more charged specific binding entities, the device being adapted to affect the electrophoretic transport of the specific binding entities in the solution to selected locations on the device surface, the device comprising:

• a substrate,
• a first electrode, the electrode being supported by the substrate, the electrode being adapted to receive current,
• a permeation layer having selective diffusion properties for the passage from the solution to the electrode of the electrolytes relative to the charged specific binding entities, permitting the free transport of counter-ions through the permeation layer to the electrode, the permeation layer being disposed adjacent the first electrode,
• an attachment layer adjacent to the permeation layer with selective binding properties for the specific binding entities, and
• an unaddressed microlocation, the first electrode being electrically insulated from the unaddressed microlocation prior to the application of the solution.