Method and device for diagnosing and distinguishing chest pain in early onset thereof
AssigneeSpectral Diagnostics, Inc.
InventorGeorge Jackowski
Life of this patent
Estimating the term…
Abstract
A diagnostic test, and a device for conducting the test, for assessing whether patient chest pain is cardiac in origin and for differentiating between unstable angina and myocardial infarction as a cause of patient chest pain is described. The diagnostic test comprises simultaneously detecting at least three selected cardiac markers with the use of at least three different monoclonal or polyclonal antibody pairs, each member of which is complementary to a different marker, which is released by heart muscle at varying stages after the onset of chest pain and is indicative of the cause of the chest pain.
Claims23
A method for determining if a patient presenting with chest pain is undergoing a cardiac event and, if so, if the cardiac event is an unstable angina or a myocardial infarction, comprising:
- A. detecting the presence of increased levels of three different markers of cardiac damage present in a blood, serum, or plasma sample from a patient after onset of chest pain, wherein:
- a. a first marker is an ischemic marker;
- b. a second marker is released from cardiac tissue only as a result of myocardial infarction;
- and
- c. a third marker is a cardiac-specific ischemic marker;
- by contacting the sample with three antibody pairs, of which one pair is specific for each of the markers and one of which antibodies from each pair is a capture antibody and the other of which is a detector antibody, whereby the presence of increased levels of a marker in the sample is indicated by binding of the marker with the respective antibody pair;
- and
- B. correlating the presence or absence of increased levels of each of the three markers with the presence or absence of unstable angina or myocardial infarction.
A diagnostic kit for determining if a patient presenting with chest pain is undergoing a cardiac event and, if so, if the cardiac event is an unstable angina or a myocardial infarction, said kit comprising:
- A. a receptacle for receiving a sample of blood, serum, plasma or a preparation thereof of the patient;
- and
- B. a detection means comprising:
- i) three antibody pairs, of which one pair is specific for each of three different markers, and one of which antibodies from each pair is a capture antibody and the other of which is a detector antibody, wherein the antibodies are selected so that:
- a. the first antibody pair binds to an ischemic marker;
- b. the second antibody pair binds to a marker released from cardiac tissue only as a result of myocardial infarction;
- and
- c. the third antibody pair binds to a cardiac-specific ischemic marker;
- and
- ii) reagents which will form a detectable product in conjunction with each of said detector antibodies to detect binding of the respective antibody pairs with their respective markers.
Dependencies are inferred from the claim text.