Printing molecular library arrays using deprotection agents solely in the vapor phase
AssigneeAffymetrix, Inc.
InventorsR. Fabian Pease, Glenn McGall, Martin J. Goldberg, Richard P. Rava, Stephen P. A. Fodor, Virginia Goss, Lubert Stryer, James L. Winkler
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Abstract
A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.
Claims24
A method of forming polymers having different monomer sequences on a substrate, said method comprising:
- providing a substrate comprising a linker molecule layer thereon, said linker molecule layer comprising a linker molecule and a protective group;
- applying a barrier layer overlying said linker molecule layer, said applying step forming selected exposed regions of said linker molecule layer;
- exposing said selected exposed regions of said linker molecule layer to a deprotecting agent solely in a vapor phase, to solely remove the protective group;
- and
- coupling selected monomers to form selected polymers on the substrate.
A method of deprotecting selected regions of a substrate, said method comprising:
- providing a substrate comprising a layer of linker molecules thereon, each of said linker molecules having a protective group;
- applying a deprotection agent solely to remove the protective group in a vapor phase to selected regions of said linker molecule layer.
A method of applying a print medium which is solely in a vapor phase in selected regions of a substrate, solely to remove a protective group, said method comprising the steps of:
- providing a partially completed substrate comprising an array of monomers, said partially completed substrate having a top surface;
- selectively applying a print medium comprising an element selected from a group consisting of a barrier material, a receptor, a deprotection agent, a monomer group, a carrier material, and an activator to selected regions of said substrate top surface.
A method of synthesizing an oligonucleotide comprising the steps of:
- coupling a first portion of said oligonucleotide to said substrate, said first portion of said oligonucleotide comprising a removable protecting group;
- solely removing said protecting group with a deprotection agent solely in a vapor phase to expose a functional group on said first portion of said oligonucleotide;
- and
- covalently bonding a second portion of said oligonucleotide to said first portion of said oligonucleotide.
Dependencies are inferred from the claim text.