US 5,599,659 A · Preservation solution for ex vivo, warm preservation of tissues, explants,organs and vascular endothelial cells comprising retinal-derived fibroblast growth factor, cyclodextrin and chondroitin sulfate

Abstract

The present invention is directed to a new hyperosmolar preservation solution useful in supporting the simultaneous in vitro growth of, and preservation of vascular endothelial cells from large vessel and microvessel origins. In addition, the preservation solution of the present invention may be used for initial flushing, and as a perfusate for storage of organs intended for transplantation using a warm preservation technology at between 18.degree. C. to 35.degree. C. Among the components of the preservation solution are colloid, mucopolysaccharide, retinal-derived fibroblast growth factor and a high magnesium concentration. Also, the present invention is directed to a method for preserving, without extreme hypothermia, an organ intended to be transplanted using the preservation solution.

Claims22

Dependent claims

A solution with an osmolarity of 330 to 600 mOsm comprising:

(a) a cell culture medium;
(b) Mg++ in a concentration greater than 10mmol/L;
and
(c) chondroitin sulfate, heparin, cyclodextrin, insulin, cholesterol, pyruvate, and retinal-derived fibroblast growth factor in concentrations sufficient to preserve tissues, explants, vascular endothelial cells, and organs at temperatures ranging from about 18.degree. C. to about 35.degree. C.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

08/372,782Filed 13 Jan 1995 · continuation in part

Priority

11 Mar 1993

Granted

4 Feb 1997

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

A01N 1/10
Preservation of bodies of humans or animals, or parts thereof: preservation of living parts
A01N 1/126
Preservation or perfusion media: physiologically active agents, e.g. antioxidants or nutrients
C12N 5/069
Vertebrate cells: vascular Endothelial cells
C12N 2500/16
Light metals, i.e. alkali, alkaline earth, Be, Al, Mg: magnesium; Mg chelators

Preservation solution for ex vivo, warm preservation of tissues, explants,organs and vascular endothelial cells comprising retinal-derived fibroblast growth factor, cyclodextrin and chondroitin sulfate

Life of this patent

Preservation solution for ex vivo, warm preservation of tissues, explants,organs and vascular endothelial cells comprising retinal-derived fibroblast growth factor, cyclodextrin and chondroitin sulfate

Life of this patent

Abstract

Claims22