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US 5,597,910 AGranted
Electrochemiluminescent label for DNA probe assays
AssigneeIgen, Inc.
InventorsSatyanarayana R. Gudibande, John H. Kenten
Life of this patent
Estimating the term…
Abstract
This invention relates to a new electrochemiluminescent (ECL) label for oligonucleotides using phosphoramidite chemistry.
Claims9
A compound of the formula:
- ##STR23## wherein M is selected from the group consisting of Ru, Os and Re;
- u.t=v, u is 1 or 2 and t is 1 or 2;
- L.sub.1, L.sub.2, and L.sub.3 are the same or not all the same and each is ##STR24## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5 and R.sub.6 are the same or not all the same and each is H, alkyl of from 1-5 carbon atoms, ##STR25## and at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5 and R.sub.6 is either ##STR26## n is an integer in the range from 1-20;
- X is selected from the group consisting of O, S, SO.sub.2, COO, and CONH;
- a and b are --N(CH--(CH.sub.3).sub.2).sub.2, --NH--CH--(CH.sub.3).sub.2, O--(CH.sub.2).sub.2 --CN, O--CH.sub.3 or morpholino, provided that a and b are not the same;
- and Z is a counterion, provided that said counterion is an anion associated with the synthesis or purification of the compound;
- and further provided that ligands L.sub.1, L.sub.2 and L.sub.3 are selected such that the compound is capable of electrochemiluminescence.
4 more elements
A compound of the formula:
- ##STR27## wherein M is selected from the group consisting of Ru, Os and Re;
- L.sub.1, L.sub.2 and L.sub.3 are the same or different and each is ##STR28## wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5 and R.sub.6 are the same or different and each is H or an alkyl group of 1-5 carbon atoms provided, however, that one of R.sub.1 -R.sub.6 is a linker of the formula ##STR29## wherein n is an integer from 1-20;
- X is selected from the group consisting of O, S, SO.sub.2, COO and CONH;
- y is an integer of from 1-20;
- z is 0 or 1;
- and B is a biomolecule or a synthetic analog thereof, and the phosphodiester group is bound to said biomolecule;
- said compound being capable of electrochemiluminescence.
4 more elements
A complex of the formula ##STR30## wherein M is ruthenium, osmium or rhenium;
- R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are the same or different and each is H or alkyl of 1-4 carbon atoms;
- n is an integer from 1 to 20;
- X is selected from the group consisting of O, S, SO.sub.2, COO and CONH;
- y is an integer of from 1-20;
- z is 0 or 1;
- m is an integer of from 1-1000;
- and NAB is a nucleic acid base which may be modified or unmodified;
- said complex being able to electrochemiluminesce.
5 more elements
Dependencies are inferred from the claim text.